MCQ Biochemical Tests


 

1.       What reagent is used in the oxidase test?

a)       Tetramethyl-p-phenylenediamine dihydrochloride

b)      Methyl red

c)       Kovac's reagent

d)      Urea broth

Answer: a

 

2.       A positive catalase test indicates the presence of:

a)       Hydrogen peroxide

b)      Catalase enzyme

c)       Indole

d)      Nitrate

Answer: b

 

3.       Which bacterium is likely to produce a positive coagulase test result?

a)       Escherichia coli

b)      Staphylococcus aureus

c)       Streptococcus pyogenes

d)      Pseudomonas aeruginosa

Answer: b

 

4.       A positive Voges-Proskauer (VP) test result is indicated by:

a)       Pink/red color change

b)      Blue color change

c)       Yellow color change

d)      No color change

Answer: a

 

5.       Which reagent is used in the urease test?

a)       Urea broth

b)      Zinc

c)       Methyl red indicator

d)      Simmons citrate agar

Answer: a

 

6.       A bacterium that produces a blue color change in Simmons citrate agar is:

a)       Citrate-positive

b)      Citrate-negative

c)       Lactose fermenter

d)      Urease-positive

Answer: a

 

7.       Which of the following organisms is likely to yield a positive nitrate reduction test result?

a)       Escherichia coli

b)      Staphylococcus aureus

c)       Pseudomonas aeruginosa

d)      Streptococcus pyogenes

Answer: a

 

8.       A bacterium that ferments glucose and lactose but not sucrose will yield which TSI test result?

a)       Red slant/yellow butt

b)      Yellow slant/red butt

c)       Red slant/red butt

d)      Yellow slant/yellow butt

Answer: b

 

9.       Which reagent is used to detect the production of acids in the Methyl Red (MR) test?

a)       Methyl red indicator

b)      VP reagents A and B

c)       Simmons citrate agar

d)      Nitrate reagents A and B

Answer: a

 

10.   The oxidase test is useful for differentiating between:

a)       Staphylococcus and Streptococcus

b)      Enterobacteriaceae and Pseudomonas

c)       Gram-positive and Gram-negative bacteria

d)      Bacillus and Clostridium

Answer: b

 

11.   Which reagent is used in the nitrate reduction test?

a)       Methyl red indicator

b)      Tetramethyl-p-phenylenediamine dihydrochloride

c)       VP reagents A and B

d)      Kovac's reagent

Answer: b

 

12.   A positive indole test result indicates the presence of:

a)       Hydrogen peroxide

b)      Indole

c)       Nitrate

d)      Catalase enzyme

Answer: b

 

13.   The Voges-Proskauer (VP) test detects the production of:

a)       Acids

b)      Butanediol

c)       Ammonia

d)      Hydrogen sulfide

Answer: b

 

14.   Which bacterium is known for producing green pigment on agar and is associated with infections in burn patients?

a)       Escherichia coli

b)      Pseudomonas aeruginosa

c)       Staphylococcus aureus

d)      Klebsiella pneumoniae

Answer: b

 

15.   A positive urease test result is indicated by:

a)       A pink/red color change

b)      Gas production

c)       A yellow color change

d)      A green color change

Answer: b

 

16.   Which of the following bacteria is most likely to yield a positive citrate utilization test result?

a)       Escherichia coli

b)      Salmonella enterica

c)       Streptococcus pneumoniae

d)      Neisseria gonorrhoeae

Answer: b

 

17.   The Methyl Red (MR) test detects the production of:

a)       Acids

b)      Alkaline products

c)       Ammonia

d)      Indole

Answer: a

 

18.   A bacterium that produces gas from glucose fermentation and hydrogen sulfide from sulfur reduction will yield which TSI test result?

a)       Red slant/yellow butt

b)      Yellow slant/red butt

c)       Yellow slant/yellow butt

d)      Red slant/red butt

Answer: b

 

19.   Which of the following organisms is likely to produce a positive citrate utilization test result?

a)       Escherichia coli

b)      Pseudomonas aeruginosa

c)       Streptococcus pyogenes

d)      Staphylococcus aureus

Answer: b

 

20.   The oxidase test is useful for differentiating between:

a)       Staphylococcus and Streptococcus

b)      Enterobacteriaceae and Pseudomonas

c)       Gram-positive and Gram-negative bacteria

d)      Bacillus and Clostridium

Answer: b

 

21.   Differential Diagnosis:

 

22.   A Gram-negative, oxidase-positive, non-lactose fermenter that produces a fruity odor and green pigment on agar likely belongs to which genus?

a)       Escherichia

b)      Klebsiella

c)       Pseudomonas

d)      Enterobacter

Answer: c

 

23.   A catalase-positive, coagulase-negative coccus that is novobiocin-resistant is likely:

a)       Staphylococcus aureus

b)      Staphylococcus epidermidis

c)       Streptococcus pyogenes

d)      Streptococcus pneumoniae

Answer: b

 

24.   Which bacterium produces indole, is lysine and ornithine decarboxylase positive, and has a "brick red" pigment on MacConkey agar?

a)       Escherichia coli

b)      Klebsiella pneumoniae

c)       Proteus mirabilis

d)      Salmonella enterica

Answer: c

 

25.   A bacterium that is citrate-negative, ferments glucose, and produces gas from lactose fermentation likely belongs to which genus?

a)       Klebsiella

b)      Proteus

c)       Salmonella

d)      Enterobacter

Answer: a

 

26.   A Gram-negative bacterium that ferments lactose with gas production, produces H2S, and is indole-negative is likely:

a)       Escherichia coli

b)      Proteus mirabilis

c)       Salmonella enterica

d)      Shigella flexneri

Answer: c

 

27.   Which of the following bacteria is known for producing a "blue-green" pigment on agar and can cause urinary tract infections?

a)       Escherichia coli

b)      Pseudomonas aeruginosa

c)       Klebsiella pneumoniae

d)      Staphylococcus aureus

Answer: b

 

28.   A Gram-positive coccus that grows in chains and is beta-hemolytic on blood agar likely belongs to which species?

a)       Staphylococcus aureus

b)      Streptococcus pyogenes

c)       Staphylococcus epidermidis

d)      Streptococcus pneumoniae

Answer: b

 

29.   A bacterium that produces H2S and causes a green metallic sheen on EMB agar is likely:

a)       Escherichia coli

b)      Proteus mirabilis

c)       Salmonella enterica

d)      Shigella flexneri

Answer: b

 

30.   A Gram-positive coccus that is catalase-negative, optochin-sensitive, and causes pneumonia is likely:

a)       Streptococcus pyogenes

b)      Streptococcus agalactiae

c)       Staphylococcus aureus

d)      Streptococcus pneumoniae

Answer: d

 

31.   Which of the following organisms is associated with "rice-water" stools and can cause severe diarrhea and dehydration?

a)       Escherichia coli

b)      Vibrio cholerae

c)       Shigella flexneri

d)      Campylobacter jejuni

Answer: b

 

32.   A bacterium that is positive for the indole test, negative for citrate utilization, and causes urinary tract infections is likely:

a)       Escherichia coli

b)      Staphylococcus aureus

c)       Streptococcus pyogenes

d)      Pseudomonas aeruginosa

Answer: a

 

33.   A catalase-positive, beta-hemolytic coccus that is bacitracin-resistant and causes pneumonia is likely:

a)       Staphylococcus aureus

b)      Streptococcus pyogenes

c)       Staphylococcus epidermidis

d)      Streptococcus pneumoniae

Answer: d

 

34.   Which of the following organisms is known for producing a "chocolate" odor and causing respiratory tract infections in cystic fibrosis patients?

a)       Staphylococcus aureus

b)      Pseudomonas aeruginosa

c)       Haemophilus influenzae

d)      Escherichia coli

Answer: b

 

35.   A bacterium that is oxidase-positive, ferments glucose, and produces a green pigment on agar is likely:

a)       Staphylococcus aureus

b)      Escherichia coli

c)       Pseudomonas aeruginosa

d)      Streptococcus pyogenes

Answer: c

 

36.   A Gram-positive coccus that is catalase-negative, coagulase-negative, and causes infections in prosthetic devices is likely:

a)       Staphylococcus aureus

b)      Staphylococcus epidermidis

c)       Streptococcus pyogenes

d)      Streptococcus pneumoniae

Answer: b

 

37.   A bacterium that produces a pink/red color change in Simmons citrate agar is likely:

a)       Citrate-positive

b)      Citrate-negative

c)       Lactose fermenter

d)      Urease-positive

Answer: a

 

38.   Which of the following organisms is associated with a red "bull's-eye" rash, joint pain, and is transmitted by ticks?

a)       Borrelia burgdorferi

b)      Treponema pallidum

c)       Neisseria gonorrhoeae

d)      Haemophilus influenzae

Answer: a

 

39.   A bacterium that is urease-positive, produces a fruity odor, and is associated with urinary tract infections is:

a)       Escherichia coli

b)      Proteus mirabilis

c)       Pseudomonas aeruginosa

d)      Staphylococcus aureus

Answer: b

 

40.   A Gram-negative, oxidase-positive, curved bacterium that causes bloody diarrhea and is associated with contaminated water is:

a)       Escherichia coli

b)      Salmonella enterica

c)       Shigella flexneri

d)      Vibrio cholerae

Answer: d

 

41.   A bacterium that is beta-hemolytic, bacitracin-sensitive, and causes "flesh-eating" infections is likely:

a)       Staphylococcus aureus

b)      Streptococcus pyogenes

c)       Staphylococcus epidermidis

d)      Streptococcus pneumoniae

Answer: b

 

42.   A bacterium that causes food poisoning due to pre-formed toxin and is associated with reheated rice is:

a)       Staphylococcus aureus

b)      Bacillus cereus

c)       Clostridium perfringens

d)      Escherichia coli

Answer: b

 

43.   Which of the following organisms is associated with "honey-crusted" lesions and is common in children?

a)       Staphylococcus aureus

b)      Streptococcus pyogenes

c)       Corynebacterium diphtheriae

d)      Mycobacterium tuberculosis

Answer: c

 

44.   A bacterium that is oxidase-negative, causes urinary tract infections, and forms pink colonies on MacConkey agar is likely:

a)       Escherichia coli

b)      Pseudomonas aeruginosa

c)       Klebsiella pneumoniae

d)      Staphylococcus aureus

Answer: a

 

45.   A Gram-negative bacterium that is oxidase-positive, motile, and causes "swarming" on agar is likely:

a)       Escherichia coli

b)      Klebsiella pneumoniae

c)       Proteus mirabilis

d)      Shigella flexneri

Answer: c

 

46.   A bacterium that is beta-hemolytic, bacitracin-sensitive, and causes pharyngitis in children is likely:

a)       Staphylococcus aureus

b)      Streptococcus pyogenes

c)       Staphylococcus epidermidis

d)      Streptococcus pneumoniae

Answer: b

 

47.   Which of the following organisms is associated with "rusty" sputum and is a common cause of community-acquired pneumonia?

a)       Mycobacterium tuberculosis

b)      Streptococcus pneumoniae

c)       Haemophilus influenzae

d)      Legionella pneumophila

Answer: b

 

48.   A Gram-positive, spore-forming bacterium that is motile and causes diarrhea is likely:

a)       Bacillus cereus

b)      Clostridium difficile

c)       Clostridium perfringens

d)      Clostridium tetani

Answer: a

 

49.   A bacterium that is catalase-negative, alpha-hemolytic, optochin-sensitive, and bile soluble likely belongs to which species?

a)       Streptococcus pyogenes

b)      Streptococcus agalactiae

c)       Staphylococcus aureus

d)      Streptococcus pneumoniae

Answer: d

 

50.   Which of the following organisms is associated with "pseudomembranous" colitis and overgrowth due to antibiotic use?

a)       Clostridium difficile

b)      Clostridium perfringens

c)       Corynebacterium diphtheriae

d)      Mycobacterium tuberculosis

Answer: a

 

51.   A Gram-negative, oxidase-positive, rod-shaped bacterium that causes a sexually transmitted infection and is associated with "strawberry cervix" is:

a)       Neisseria gonorrhoeae

b)      Chlamydia trachomatis

c)       Treponema pallidum

d)      Haemophilus ducreyi

Answer: a

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