1. Which
fixative is commonly used for preserving tissue samples in histopathology?
a) Formalin
b) Acetone
c) Ethanol
d) Xylene
2. What
is the purpose of tissue processing in histopathology?
a) Remove
excess water from the tissue
b) Harden
the tissue for sectioning
c) Stain
the tissue for visualization
d) Fix
the tissue to prevent degradation
3. What
is the optimal temperature for embedding tissue in paraffin wax?
a) 4°C
b) 25°C
c) 37°C
d) 60°C
4. Which
instrument is used for cutting thin sections of tissue in histopathology?
a) Microtome
b) Centrifuge
c) Cryostat
d) Autoclave
5. What
is the purpose of deparaffinization in histopathology?
a) Remove
excess water from the tissue
b) Remove
paraffin from the tissue sections
c) Enhance
the staining of tissue components
d) Prevent
tissue damage during staining
6. Which
stain is commonly used to visualize nuclei in histopathology?
a) Hematoxylin
b) Eosin
c) Giemsa
d) Masson's
trichrome
7. What
is the function of counterstaining in histopathology staining techniques?
a) Differentiate
between cell types
b) Enhance
the visibility of specific tissue components
c) Remove
excess stain from the tissue sections
d) Provide
contrast to the primary stain
8. Which
staining technique is commonly used to identify microorganisms in
histopathology?
a) Gram
stain
b) Periodic
acid-Schiff (PAS) stain
c) Ziehl-Neelsen
stain
d) Wright's
stain
9. Which
type of antibody is used in immunohistochemistry (IHC) to detect antigens in
tissue sections?
a) Primary
antibody
b) Secondary
antibody
c) Polyclonal
antibody
d) Monoclonal
antibody
10. What
is the purpose of mounting media in histopathology?
a) Preserve
the tissue sections
b) Enhance
the clarity of microscopic images
c) Remove
excess stain from the tissue sections
d) Prevent
tissue damage during staining
11. Which
staining technique is commonly used to demonstrate reticular fibers in
histopathology?
a) Periodic
acid-Schiff (PAS) stain
b) Masson's
trichrome stain
c) Gomori's
silver stain
d) Alcian
blue stain
12. What
is the function of antigen retrieval in immunohistochemistry (IHC)?
a) Amplify
the signal from the antibody
b) Remove
unwanted tissue components
c) Enhance
the penetration of antibodies into the tissue
d) Prevent
tissue damage during staining
13. Which
stain is commonly used to visualize amyloid deposits in histopathology?
a) Congo
red
b) Wright's
stain
c) Toluidine
blue
d) Prussian
blue
14. What
is the primary stain used in the Papanicolaou (Pap) stain for cytology specimens?
a) Hematoxylin
b) Eosin
c) Papanicolaou
red
d) Methylene
blue
15. Which
of the following is an example of a cytological sample suitable for Pap
staining?
a) Biopsy
specimen
b) Blood
smear
c) Fine
needle aspiration (FNA) sample
d) Excised
tumor tissue
16. What
is the purpose of the Sudan Black B stain in histopathology?
a) Visualize
lipids in tissue sections
b) Detect
iron deposits in tissue sections
c) Stain
nucleoli in tissue sections
d) Identify
specific cell types in tissue sections
17. Which
type of microscopy is commonly used to examine unstained, live cells in
histopathology?
a) Brightfield
microscopy
b) Fluorescence
microscopy
c) Electron
microscopy
d) Confocal
microscopy
18. Which
stain is commonly used to visualize mast cells in histopathology?
a) Giemsa
b) Periodic
acid-Schiff (PAS)
c) Prussian
blue
d) Toluidine
blue
19. What
is the purpose of mounting a coverslip on tissue sections?
a) Protect
the tissue sections from damage
b) Provide
a flat surface for microscopic observation
c) Enhance
the adherence of stain to the tissue sections
d) Prevent
evaporation of the mounting media
20. Which
of the following staining techniques is commonly used to demonstrate glycogen
in histopathology?
a) Periodic
acid-Schiff (PAS) stain
b) Masson's
trichrome stain
c) Ziehl-Neelsen
stain
d) Wright's
stain
21. Which
of the following is a characteristic feature of a well-fixed tissue specimen?
a) Cytoplasmic
vacuolation
b) Nuclear
enlargement
c) Cellular
shrinkage
d) Increased
cellular staining
22. What
is the purpose of the decalcification process in histopathology?
a) Remove
excess water from the tissue
b) Remove
calcium deposits from bone tissue
c) Harden
the tissue for sectioning
d) Fix
the tissue to prevent degradation
23. Which
stain is commonly used to visualize fungal elements in histopathology?
a) Gomori's
methenamine silver (GMS) stain
b) Gram
stain
c) Hematoxylin
and eosin (H&E) stain
d) Wright's
stain
24. What
is the purpose of the control section in immunohistochemistry (IHC)?
a) Evaluate
the staining quality
b) Provide
a reference for staining intensity
c) Determine
the specificity of the antibody
d) Identify
the target antigen in the tissue
25. Which
of the following is a feature of well-oriented tissue sections in
histopathology?
a) Tissue
folding
b) Tissue
fragmentation
c) Nuclear
overlapping
d) Uniform
thickness throughout the section
26. Which
staining technique is commonly used to demonstrate eosinophils in
histopathology?
a) Wright's
stain
b) Masson's
trichrome stain
c) Alcian
blue stain
d) Periodic
acid-Schiff (PAS) stain
27. What
is the function of a coverslip in histopathology?
a) Prevent
contamination of the tissue sections
b) Provide
a rigid support for the tissue sections
c) Enhance
the visibility of tissue components
d) Protect
the tissue sections from damage
28. Which
staining technique is commonly used to demonstrate basement membranes in
histopathology?
a) Periodic
acid-Schiff (PAS) stain
b) Gomori's
silver stain
c) Ziehl-Neelsen
stain
d) Wright's
stain
29. What
is the purpose of antigen blocking in immunohistochemistry (IHC)?
a) Prevent
nonspecific binding of antibodies
b) Enhance
the binding of primary antibodies
c) Increase
the sensitivity of the staining process
d) Improve
the signal-to-noise ratio
30. Which
type of microscopy provides three-dimensional imaging of tissue sections in
histopathology?
a) Brightfield
microscopy
b) Fluorescence
microscopy
c) Electron
microscopy
d) Confocal
microscopy
31. What
is the primary purpose of frozen section analysis in histopathology?
a) Determine
the tissue architecture
b) Assess
the tissue's response to stains
c) Provide
rapid intraoperative diagnosis
d) Examine
cellular details in real-time
32. Which
stain is commonly used to visualize bacteria in histopathology?
a) Gram
stain
b) Periodic
acid-Schiff (PAS) stain
c) Ziehl-Neelsen
stain
d) Wright's
stain
33. What
is the primary purpose of special staining techniques in histopathology?
a) Enhance
the visualization of specific tissue components
b) Remove
unwanted artifacts from tissue sections
c) Improve
the overall quality of staining
d) Prevent
tissue damage during the staining process
34. Which
of the following is a characteristic feature of a well-prepared frozen section
slide?
a) Cellular
distortion
b) Cellular
shrinkage
c) Cellular
folding
d) Minimal
ice crystal artifacts
35. Which
stain is commonly used to visualize melanin pigment in histopathology?
a) Hematoxylin
b) Eosin
c) Fontana-Masson
stain
d) Alcian
blue
36. What
is the purpose of the xylene clearing step in histopathology staining techniques?
a) Remove
excess stain from the tissue sections
b) Enhance
the visibility of tissue components
c) Remove
paraffin from the tissue sections
d) Provide
contrast to the primary stain
37. Which
staining technique is commonly used to demonstrate iron deposits in
histopathology?
a) Prussian
blue stain
b) Masson's
trichrome stain
c) Periodic
acid-Schiff (PAS) stain
d) Wright's
stain
38. What
is the primary function of mounting media in histopathology?
a) Remove
excess water from the tissue sections
b) Enhance
the clarity of microscopic images
c) Remove
excess stain from the tissue sections
d) Prevent
tissue damage during the staining process
39. Which
of the following is an example of a fixative used for electron microscopy in
histopathology?
a) Formalin
b) Acetone
c) Glutaraldehyde
d) Xylene
40. What
is the purpose of the nuclear fast red counterstain in histopathology?
a) Enhance
the visualization of nuclei
b) Provide
contrast to the primary stain
c) Remove
excess stain from the tissue sections
d) Stain
specific tissue components
41. Which
staining technique is commonly used to demonstrate mucin in histopathology?
a) Alcian
blue stain
b) Periodic
acid-Schiff (PAS) stain
c) Ziehl-Neelsen
stain
d) Wright's
stain
42. What
is the primary function of an antibody in immunohistochemistry (IHC)?
a) Stain
tissue sections for visualization
b) Enhance
the clarity of microscopic images
c) Detect
specific antigens in tissue sections
d) Remove
unwanted tissue components
43. Which
stain is commonly used to visualize fibrin in histopathology?
a) Masson's
trichrome stain
b) Hematoxylin
c) Eosin
d) Gomori's
silver stain
44. What
is the purpose of the washing step in histopathology staining techniques?
a) Remove
excess stain from the tissue sections
b) Enhance
the visibility of tissue components
c) Remove
unwanted artifacts from tissue sections
d) Provide
contrast to the primary stain
45. Which
staining technique is commonly used to demonstrate elastic fibers in
histopathology?
a) Verhoeff's
stain
b) Periodic
acid-Schiff (PAS) stain
c) Ziehl-Neelsen
stain
d) Wright's
stain
46. What
is the function of the control tissue section in immunohistochemistry (IHC)?
a) Evaluate
the staining quality
b) Provide
a reference for staining intensity
c) Determine
the specificity of the antibody
d) Identify
the target antigen in the tissue
47. Which
of the following is a characteristic feature of well-stained tissue sections in
histopathology?
a) Cytoplasmic
vacuolation
b) Nuclear
enlargement
c) Intense
cellular staining
d) Cellular
shrinkage
48. Which
staining technique is commonly used to demonstrate acid-fast bacteria in
histopathology?
a) Ziehl-Neelsen
stain
b) Periodic
acid-Schiff (PAS) stain
c) Wright's
stain
d) Alcian
blue stain
49. What
is the purpose of the Sudan III stain in histopathology?
a) Visualize
lipids in tissue sections
b) Detect
iron deposits in tissue sections
c) Stain
nucleoli in tissue sections
d) Identify
specific cell types in tissue sections
50. Which
type of microscopy is commonly used to examine fluorescently labeled tissue
sections in histopathology?
a) Brightfield
microscopy
b) Fluorescence
microscopy
c) Electron
microscopy
d) Confocal
microscopy
1 a 11 c 21 c 31 c 41 a
2 b 12 c 22 b 32 a 42 c
3 d 13 a 23 a 33 a 43 a
4 a 14 c 24 b 34 d 44 a
5 b 15 c 25 d 35 c 45 a
6 a 16 a 26 a 36 c 46 c
7 d 17 b 27 c 37 a 47 c
8 a 18 a 28 a 38 b 48 a
9 a 19 b 29 a 39 c 49 a
10 b 20 a 30 b 40 a 50 b